目的：观察消白灵汤对体外培养小鼠黑素细胞的影响，探讨消白灵汤治疗白癜风的作用机制。方法：以1640培养基体外培养B-16F10小鼠黑素瘤细胞，分别加入不同浓度(1mg/ml、2mg/ml、3mg/ml)的中药消白灵汤及其主要组分——温补肾阳药、养血活血药等，作用7天。采用MTT法测定B-16F10小鼠黑素瘤细胞增殖情况，采用NaOH裂解法测定黑素细胞黑素生成量。结果：不同浓度的消白灵汤、温补肾阳药及养血活血药从第3天至第5天均可显著促进B-16F10小鼠黑素瘤细胞增殖(P<0.05)，其中1mg/ml消白灵汤在第3天对黑素细胞增殖的促进作用最为明显(P<0.001);2mg/ml、3mg/ml温补肾阳药及3mg/ml养血活血药可显著增加黑素细胞黑素生成量(P<0.05)。结论：消白灵汤可在体外促进黑素细胞增殖及黑素生成，提示温补肾阳药和养血活血药在治疗白癜风中发挥重要作用。

To observe the effect of Xiaobailing Decoction(消白灵汤)on murine melanocytes in vitro and to explore the mechanism of Xiaobailing Decoction in the treatment of vitiligo. Methods: B-16F10 murine melanoma cells were cultured in 1640 medium and treated respectively with different concentrations (1mg/ml, 2mg/ml, 3mg/ml) of the Chinese drug Xiaobailing Decoction and its maincomponents, the drugs for replenishing the kidney-yang, and the drugs for nourishing blood and activatingblood circulation, etc. for 7 days. MMT assay was used to determine the proliferation of B-16F10 murine melanoma cells. NaOH cleavage assay was adopted to measure the melanogenesis of melanocytes. Results:Xiaobailing Decoction, the drugs for replenishing the kidney-yang and the drugs for nourishing blood and activating blood circulation at different concentrations significantly improved the proliferation of B-16F10 murine melanoma cells from the 3rd day to the 5th day (P<0.05), with Xiaobailing Decoction at the concentrations of 1mg/ml having the most distinct action on promoting the proliferation of melanocytes on the 3rd day (P<0.001); And the drugs for replenishing the kidney-yang at the concentrations of 2mg/ml and 3mg/ml and the drugs for nourishing blood and activating blood circulation at 3mg/ml significantly increased melanogenesis of melanocytes (P<0.05). Conclusion: Xiaobailing Decoction can promote melanocytic proliferation and melanogenesis in vitro, and it is indicated that the drugs for replenishing the kidney-yang and the drugs for nourishing blood and activating blood circulation play an important role in treating vitiligo.